In the U.S. and Canada it was 2 9/16 inch. In Europe it was 65mm or 2 1/2 inch. While the "standard" length North American 20-gauge shell was 2 1/2 inch, when Western Cartridge Co. brought out their 20-gauge Super-X shell in 1922, they put it up in a 2 3/4 inch case. The next year when they brought out their 16-gauge Super-X shell it stayed in the "standard" 2 9/16 inch case with a load of 3 drams equiv. and 1 1/8 ounce of shot. Remington followed suit with their Nitro Express. In 1931, when Remington Arms Co., Inc. added the 16-gauge their John M. Browning designed autoloaders, the Model 11 and The Sportsman, the gun was made for 2 3/4 inch shells. Remington added a 2 3/4 inch 16-gauge shell to their line called the Auto Express with a load of 3 1/4 drams equiv. and 1 1/8 ounces of shot.
The one thing that seems confusing in this is that in 1926, Winchester lengthened the chambers and ejection ports of their Model 12s in both 16- and 20-gauge to take 2 3/4 inch shells. It makes sense in the 20-gauge to compete with Remington's Model 17 which was made for 2 3/4 inch 20-gauge shells from the get go. At that time the only 2 3/4 inch 16-gauge shells offered in the catalogues were older style shells like the old Winchester Leader that were offered in extra length. But, they were loaded with the older bulk or dense smokeless powders and carried a maximum load of 1 ounce, while the 2 9/16 inch Super-X/Nitro Express type shells loaded with progressive burning powder carried 1 1/8 ounce?!?
In their 1934 catalogues, Ithaca Gun Co. began stating that their 16-gauge guns were chambered for 2 3/4 inch shells. Savage in their Fox catalogues didn't begin mentioning 2 3/4 inch chambering until their 1938 catalogue.